Part:BBa_K2073013:Experience

Applications of BBa_K2073013

Firstly, we aimed to know the effect of fluoride on E.coli. We cultured E.coli in LB with different sodium fluoride concentration and traced the growth curve of E.coli in fifteen hours.

The result shows that fluoride significantly inhibits the growth of E.coli. The higher fluoride concentration , the less E.coli grow.
In the LB with 0.1M sodium fluoride, the E.coli stops growing.


Secondly, we tested the protein function of Crcb-La2. After transfecting Crcb-La2 gene into E.coli, we cultured them in the LB with 0.1M sodium fluoride.
The positive control is E.coli cultured in LB without sodium fluoride.
The negative control is E.coli without Crcb-La2 gene cultured in LB with 0.1M sodium fluoride

Based on the result, in the LB with 0.1M sodium fluoride, the E.coli with Crcb-La2 gene can still grow. (Crcb-La2-test 1,2) However, as for the E.coli without Crcb-La2 gene, the growth is completely inhibited.(negative control)
We can know the Crcb-La2 successfully exfluxes the fluoride and promotes the growth of E.coli.

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